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PPP1R12A Rabbit pAb (A0587)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PPP1R12A Rabbit pAb (A0587)

Western blot analysis of various lysates using PPP1R12A Rabbit pAb (A0587) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of HeLa cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of NIH/3T3 cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of PC-12 cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of U2OS cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PPP1R12A Rabbit pAb (A0587)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg PPP1R12A antibody (A0587). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (A0587) at a dilution of 1:1000.

Overview

Product namePPP1R12A Rabbit pAb
Catalog No.A0587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
SequenceMKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGG
Gene ID
Swiss Prot
SynonymsMBS; GUBS; M130; MYPT1; PPP1R12A
Calculated MW115kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, LO2, Jurkat, HeLa
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - PPP1R12A Rabbit pAb (A0587)}

Western blot - PPP1R12A Rabbit pAb (A0587)

Western blot analysis of various lysates using PPP1R12A Rabbit pAb (A0587) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)}

Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of HeLa cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)}

Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of NIH/3T3 cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)}

Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of PC-12 cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PPP1R12A Rabbit pAb (A0587)}

Immunofluorescence - PPP1R12A Rabbit pAb (A0587)

Immunofluorescence analysis of U2OS cells using PPP1R12A Rabbit pAb (A0587) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PPP1R12A Rabbit pAb (A0587)}

Immunoprecipitation - PPP1R12A Rabbit pAb (A0587)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg PPP1R12A antibody (A0587). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (A0587) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

Secondary Antibodies (26)