제품 > 항체 > 다중클론항체(pAb)

PTEN Rabbit pAb (A11193)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PTEN Rabbit pAb (A11193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PTEN Rabbit pAb (A11193)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using PTEN Rabbit pAb (A11193) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human placenta using PTEN Rabbit pAb (A11193) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using PTEN Rabbit pAb (A11193) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat testis using PTEN Rabbit pAb (A11193) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PTEN Rabbit pAb (A11193)

Immunofluorescence analysis of U2OS cells using PTEN Rabbit pAb (A11193) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	PTEN Rabbit pAb
Catalog No.	A11193
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PTEN (NP_000305.3).
Sequence	MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMF
Gene ID
Swiss Prot
Synonyms	BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; PTEN
Calculated MW	47kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3, C6
Cellular location	Cytoplasm, Nucleus, PML body, Secreted
Customer validation	(H460) WB(Homo sapiens, Mus musculus, Capra hircus, Ctenopharyngodon idellus, Mus musculus, Rattus norvegicus) IHC(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

PTEN Rabbit pAb images

Western blot - PTEN Rabbit pAb (A11193)

Immunohistochemistry - PTEN Rabbit pAb (A11193)

Immunofluorescence - PTEN Rabbit pAb (A11193)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11193

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Reviews List

Product Protocols

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Mediators of Inflammation

Application:

IF:

4.711

Species:

Homo sapiens

PMID:

29118466

Title:

Associations of Functional MicroRNA Binding Site Polymorphisms in IL23/Th17 Inflammatory Pathway Genes with Gastric Cancer Risk

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Acta Histochemica

Application:

IF:

2.479

Species:

Mus musculus

PMID:

32183989

Title:

β-elemene suppresses the malignant behavior of esophageal cancer cells by regulating the phosphorylation of AKT

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Mol Med Rep

Application:

IF:

2.952

Species:

Mus musculus

PMID:

32901892

Title:

miR‑183‑5p attenuates cerebral ischemia injury by negatively regulating PTEN

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

J Anim Sci Biotechnol

Application:

IF:

5.032

Species:

Capra hircus

PMID:

33072314

Title:

Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Environ Pollut

Application:

IF:

8.07

Species:

Ctenopharyngodon idellus

PMID:

34488159

Title:

Quercetin antagonizes imidacloprid-induced mitochondrial apoptosis through PTEN/PI3K/AKT in grass carp hepatocytes

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Mol Ther Nucleic Acids

Application:

IF:

8.88

Species:

Mus musculus

PMID:

32585629

Title:

miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Ecotoxicol Environ Saf

Application:

IF:

6.291

Species:

Mus musculus

PMID:

34271501

Title:

Copper exposure induces hepatic G0/G1 cell-cycle arrest through suppressing the Ras/PI3K/Akt signaling pathway in mice

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Neoplasma

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

32122140

Title:

Inhibition of miR-103a-3p suppresses the proliferation in oral squamous cell carcinoma cells via targeting RCAN1

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Journal of oncology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

36213832

Title:

Effect of Long Noncoding RNA HULC on Proliferation, Migration, and Invasion of Osteosarcoma Cells - PMC

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.75

Species:

Mus musculus

PMID:

37217071

Title:

AFP deletion leads to anti-tumorigenic but pro-metastatic roles in liver cancers with concomitant CTNNB1 mutations

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Homo sapiens

PMID:

37483811

Title:

Knockdown of FUT11 inhibits the progression of gastric cancer via the PI3K/AKT pathway - PMC

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Tissue & cell

Application:

IF:

2.58

Species:

Homo sapiens

PMID:

37865041

Title:

Mitochondrial transplantation inhibits cholangiocarcinoma cells growth by balancing oxidative stress tolerance through PTEN/PI3K/AKT signaling pathway

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Parasites & vectors

Application:

IF:

4.04

Species:

Homo sapiens, Mus musculus

PMID:

38124152

Title:

csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis - PMC

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

WB, IHC, IF

IF:

Species:

Mus musculus

PMID:

35780838

Title:

A WWP2-PTEN-KLF5 signaling axis regulates odontoblast differentiation and dentinogenesis in mice - PMC

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Cytokine

Application:

IF:

3.926

Species:

Rattus norvegicus

PMID:

29549724

Title:

Berberine inhibits IL-21/IL-21R mediated inflammatory proliferation of fibroblast-like synoviocytes through the attenuation of PI3K/Akt signaling pathway and

References for PTEN Rabbit pAb (A11193)

Product:

PTEN Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.4

Species:

Homo sapiens, Mus musculus

PMID:

31560953