제품 > 항체 > 다중클론항체(pAb)

Pan-Akt Rabbit pAb (A18120)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Pan-Akt Rabbit pAb (A18120) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Pan-Akt Rabbit pAb (A18120)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using Pan-Akt Rabbit pAb (A18120) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Pan-Akt Rabbit pAb (A18120) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Pan-Akt Rabbit pAb (A18120)

Immunofluorescence analysis of C6 cells using Pan-Akt Rabbit pAb (A18120) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using Pan-Akt Rabbit pAb (A18120) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Pan-Akt Rabbit pAb (A18120) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Pan-Akt Rabbit pAb (A18120)

Immunoprecipitation analysis of 25 μg extracts of Rat brain cells using 3 μg Pan-Akt antibody (A18120). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (A18120) at a dilution of 1:1000.

Overview

Product name	Pan-Akt Rabbit pAb
Catalog No.	A18120
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Human AKT serine-threonine protein kinase family includes three members AKT1, AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4, 5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 311-480 of human AKT1/AKT2/AKT3 (NP_005154.2).
Sequence	GTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Gene ID
Swiss Prot
Synonyms	AKT1/AKT2/AKT3; Pan-Akt
Calculated MW	48kDa/55kDa/51kDa/54kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	MCF7, Mouse brain, Rat brain
Cellular location	ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle.
Customer validation	IF(Homo sapiens) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Mus musculus, Sus scrofa)

Documents

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Research Area

Pan-Akt Rabbit pAb images

Western blot - Pan-Akt Rabbit pAb (A18120)

Immunohistochemistry - Pan-Akt Rabbit pAb (A18120)

Immunofluorescence - Pan-Akt Rabbit pAb (A18120)

Immunoprecipitation - Pan-Akt Rabbit pAb (A18120)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Molecules

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

33143000

Title:

The Synthetic Flavonoid Derivative GL-V9 Induces Apoptosis and Autophagy in Cutaneous Squamous Cell Carcinoma via Suppressing AKT-Regulated HK2 and mTOR Signals

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

34153312

Title:

MicroRNA-23b attenuates tau pathology and inhibits oxidative stress by targeting GnT-III in Alzheimer's disease

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Front Oncol

Application:

IF:

3.5

Species:

Mus musculus

PMID:

34790577

Title:

Anti-Tumor Activity of AZD4547 Against NTRK1 Fusion Positive Cancer Cells Through Inhibition of NTRKs

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Mus musculus

PMID:

36332390

Title:

Carthami flos extract against carbon tetrachloride-induced liver fibrosis via alleviating angiogenesis in mice

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

36518663

Title:

Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Cancers

Application:

IF:

5.2

Species:

Homo sapiens

PMID:

36612139

Title:

Identification of Thiazolo[5,4-b]pyridine Derivatives as c-KIT Inhibitors for Overcoming Imatinib Resistance - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.7

Species:

Mus musculus

PMID:

36563293

Title:

Novel Pro-myogenic Factor Neoruscogenin Induces Muscle Fiber Hypertrophy by Inhibiting MSTN Maturation and Activating the Akt/mTOR Pathway

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

37764863

Title:

Chinese Sumac Fruits (Rhus chinesis Mill.) Alleviate Type 2 Diabetes in C57BL/6 Mice through Repairing Islet Cell Functions, Regulating IRS-1/PI3K/AKT Pathways and Promoting the Entry of Nrf2 into the Nucleus - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Journal of Cancer

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

37781082

Title:

PRMT7 Inhibits the Proliferation and Migration of Gastric Cancer Cells by Suppressing the PI3K/AKT Pathway via PTEN

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.2

Species:

Homo sapiens

PMID:

37295712

Title:

A fluorene derivative inhibits human hepatocellular carcinoma cells by ROS-mediated apoptosis, anoikis and autophagy

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Tissue & cell

Application:

IF:

2.7

Species:

Homo sapiens

PMID:

37865041

Title:

Mitochondrial transplantation inhibits cholangiocarcinoma cells growth by balancing oxidative stress tolerance through PTEN/PI3K/AKT signaling pathway

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Current medicinal chemistry

Application:

IF:

3.5

Species:

Mus musculus

PMID:

37936460

Title:

Anti-migratory Properties of Cryoprotective Isoliquiritigenin-zein Phosphatidylcholine Nanoparticles Prevent Triple-negative Breast Cancer through PI3K-mTOR and

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Aging

Application:

IF:

3.9

Species:

Rattus norvegicus

PMID:

37610708

Title:

Gastrodin ameliorates cognitive dysfunction in diabetes by inhibiting PAK2 phosphorylation - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

38029323

Title:

Diosmin Promotes Myogenesis via Activating the Akt/FOXO1 Pathway to Facilitate the Proliferation of C2C12 Myoblasts

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Discover. Oncology

Application:

IF:

Species:

Homo sapiens

PMID:

38514510

Title:

Mechanism of regulation of KIF23 on endometrial cancer cell growth and apoptosis.

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

30798160

Title:

Magnolol prevents ossified tendinopathy by inhibiting PGE2-induced osteogenic differentiation of TDSCs.

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

35893885

Title:

The Antidiabetic Activities of Neocryptotanshinone: Screened by Molecular Docking and Related to the Modulation of PTP1B - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

35679671

Title:

Promotion Effect of Angelica Sinensis Extract on Angiogenesis of Chicken Preovulatory Follicles in Vitro

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Journal of translational medicine

Application:

IF:

8.4

Species:

Mus musculus

PMID:

38609979

Title:

Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinsons disease - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Experimental and therapeutic medicine

Application:

IF:

2.4

Species:

Rattus norvegicus

PMID:

37614421

Title:

Deciphering the role of TNF--induced protein 8-like 2 in the pathogenesis of necrotizing enterocolitis in neonatal rats - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Translational research : the journal of laboratory and clinical medicine

Application:

IF:

6.4

Species:

Homo sapiens

PMID:

38548174

Title:

Endothelial ELABELA improves post-ischemic angiogenesis by upregulating VEGFR2 expression

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Cancer cell international

Application:

IF:

6.4

Species:

Homo sapiens

PMID:

39097730

Title:

DDX19A promotes gastric cancer cell proliferation and migration by activating the PI3K/AKT pathway - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Homo sapiens, Mus musculus

PMID:

31560953

Title:

GL-V9 exerts anti-T cell malignancies effects via promoting lysosome-dependent AKT1 degradation and activating AKT1/FOXO3A/BIM axis.

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.6

Species:

Homo sapiens,Rattus norvegicus

PMID:

38562460

Title:

Isoquercitrin alleviates pirarubicin-induced cardiotoxicity in vivo and in vitro by inhibiting apoptosis through Phlpp1/AKT/Bcl-2 signaling pathway - PMC

References for Pan-Akt Rabbit pAb (A18120)

Product:

Pan-Akt Rabbit pAb

Journal:

Autophagy

Application:

IF:

14.6

Species:

Homo sapiens,Sus scrofa

PMID:

38516932