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Pan-Symmetric Di-Methyl Arginine Motif Rabbit mAb (A20794)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Pan-Symmetric Di-Methyl Arginine Motif Rabbit mAb (A20794)

Western blot analysis of various lysates, using Pan-SymmetricDi-MethylArginineMotif Rabbit mAb (A20794) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePan-Symmetric Di-Methyl Arginine Motif Rabbit mAb
Catalog No.A20794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51197
Symmetric Dimethylarginine is a dimethylated derivative of L-arginine where the two methyl groups are attached to arginine in a symmetrical configuration. Symmetric dimethylarginine (SDMA) is formed when S-adenosylmethionine protein N-methyltransferases transfer one methyl group from S-adenosylmethionine to each of the two guanidine nitrogen groups of a single arginine residue in a protein. SDMA is released when the protein is degraded. SDMA doesn't bind nitric oxide synthase (NOS) but may noncompetitivelty inhibit nitric oxide (NO) synthesis by reducing L-arginine availability; it also may play a role in the modulation of cardiovascular homeostasis and renal function.
ImmunogenA synthetic peptide corresponding to a sequence containing Symmetric Di-Methyl R.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW10-180kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HCT 116, MCF7, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular location
Customer validation

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Pan-Symmetric Di-Methyl Arginine Motif Rabbit mAb (A20794)}

    Western blot - Pan-Symmetric Di-Methyl Arginine Motif Rabbit mAb (A20794)

    Western blot analysis of various lysates, using Pan-SymmetricDi-MethylArginineMotif Rabbit mAb (A20794) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20794? Please let us know so that we can cite the reference in this datasheet.