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Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410PM)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from A-431 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410PM) at 1:2000 dilution incubated overnight at 4℃. A431 cells were treated by λ-PP mixed solution (1μl) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410PM) at 1:2000 dilution incubated overnight at 4℃. RAW 264.7 cells were treated by oligomycin (0.5 μM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410PM)

Confocal imaging of RAW 264.7 cells using Phospho-ACC1/ACC2-S79 Rabbit PolymAb® (AP1410PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	Phospho-ACC1/ACC2-S79 Rabbit PolymAb®
Catalog No.	AP1410PM
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S79 of human Acetyl CoA Carboxylase.
Sequence	SSMSG
Gene ID
Swiss Prot
Synonyms	ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha; Phospho-Acetyl CoA Carboxylase-S79
Calculated MW	265kDa
Observed MW	266kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:8000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	RAW 264.7, A-431
Cellular location	Cell membrane, Membrane raft.