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Phospho-CREB1-S133 Rabbit pAb (AP0019)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-CREB1-S133 Rabbit pAb (A11064).HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-CREB1-S133 Rabbit pAb (AP0019) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 Rabbit pAb (AP0019). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-CREB1-S133 Rabbit pAb
Catalog No.	AP0019
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).
Sequence	RPSYR
Gene ID
Swiss Prot
Synonyms	CREB; CREB-1; Phospho-CREB1-S133
Calculated MW	35kDa
Observed MW	43kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:200 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa treated by Anisomycin
Cellular location	Nucleus
Customer validation	WB(Homo sapiens, Danio rerio, Rattus norvegicus, Mus musculus, Gallus gallus) IHC(Rattus norvegicus)

Documents

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Research Area

Phospho-CREB1-S133 Rabbit pAb images

Western blot - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunohistochemistry - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunofluorescence - Phospho-CREB1-S133 Rabbit pAb (AP0019)

Immunofluorescence analysis of MCF-7 cells using Phospho-CREB1-S133 Rabbit pAb (AP0019). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Frontiers in molecular neuroscience

Application:

IF:

5.639

Species:

Homo sapiens

PMID:

29018329

Title:

CDDO and ATRA Instigate Differentiation of IMR32 Human Neuroblastoma Cells

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

6.953

Species:

Danio rerio

PMID:

30414415

Title:

Anti-melanogenesis of novel kojic acid derivatives in B16F10 cells and zebrafish.

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

5.046

Species:

Rattus norvegicus

PMID:

30607811

Title:

MALAT1 Up-Regulator Polydatin Protects Brain Microvascular Integrity and Ameliorates Stroke Through C/EBPβ/MALAT1/CREB/PGC-1α/PPARγ Pathway.

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Rattus norvegicus

PMID:

31369718

Title:

Over-Expression of TRPC6 via CRISPR Based Synergistic Activation Mediator in BMSCs Ameliorates Brain Injury in a Rat Model of Cerebral Ischemia/Reperfusion.

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Mus musculus

PMID:

31281493

Title:

Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Oxidative Medicine and Cellular Longevity

Application:

IF:

6.543

Species:

Mus musculus

PMID:

32148659

Title:

Resveratrol Mitigates Sevoflurane-Induced Neurotoxicity by the SIRT1-Dependent Regulation of BDNF Expression in Developing Mice

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

31695614

Title:

Cerebrolysin Ameliorates Focal Cerebral Ischemia Injury Through Neuroinflammatory Inhibition via CREB/PGC-1α Pathway.

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Rattus norvegicus

PMID:

34646421

Title:

Jie-Yu-He-Huan Capsule Ameliorates Anxiety-Like Behaviours in Rats Exposed to Chronic Restraint Stress via the cAMP/PKA/CREB/BDNF Signalling Pathway

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Gene

Application:

IF:

3.688

Species:

Gallus gallus

PMID:

34902510

Title:

WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Microbiol Spectr

Application:

IF:

7.171

Species:

Mus musculus

PMID:

34756071

Title:

Japanese Encephalitis Virus NS1′ Protein Interacts with Host CDK1 Protein to Regulate Antiviral Response

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Discover. Oncology

Application:

IF:

Species:

Homo sapiens

PMID:

36735162

Title:

Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis - PMC

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Metabolism: clinical and experimental

Application:

IF:

13.93

Species:

Mus musculus

PMID:

36470472

Title:

FAM3A maintains metabolic homeostasis by interacting with F1-ATP synthase to regulate the activity and assembly of ATP synthase

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Andrology

Application:

IF:

4.45

Species:

Rattus norvegicus

PMID:

36762774

Title:

Low‐intensity pulsed ultrasound ameliorates erectile dysfunction induced by bilateral cavernous nerve injury through enhancing schwann cell‐mediated cavernous …

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

6.06

Species:

Mus musculus

PMID:

37089558

Title:

Gut microbiota from sigma-1 receptor knockout mice induces depression-like behaviors and modulates the cAMP/CREB/BDNF signaling pathway - PMC

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Cell metabolism

Application:

IF:

31.37

Species:

Mus musculus

PMID:

37480842

Title:

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

37678029

Title:

Inflammatory regulation by restraining M2 microglial polarization: Neurodestructive effects of Kallikrein-related peptidase 8 activation in intracerebral hemorrhage

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Homo sapiens

PMID:

38113631

Title:

Berberine exerts antidepressant effects in vivo and in vitro through the PI3K/AKT/CREB/BDNF signaling pathway

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Neural plasticity

Application:

IF:

3.14

Species:

Rattus norvegicus

PMID:

36910013

Title:

Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation - PMC

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IHC

IF:

6.2

Species:

Rattus norvegicus

PMID:

37629166

Title:

Interleukin 6 (IL-6) Regulates GABAA Receptors in the Dorsomedial Hypothalamus Nucleus (DMH) through Activation of the JAK/STAT Pathway to Affect Heart Rate Variability in Stressed Rats - PMC

References for Phospho-CREB1-S133 Rabbit pAb (AP0019)

Product:

Phospho-CREB1-S133 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.4

Species:

Mus musculus

PMID:

38296172