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Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells, using Phospho-EGFR-Y1068 Rabbit pAb (A11351). HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes or treated by ATP(5 mM) at 30℃ for 1 hour after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review

Overview

Product name	Phospho-EGFR-Y1068 Rabbit pAb
Catalog No.	AP0301
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1068 of human EGFR (NP_005219.2).
Sequence	PEYIN
Gene ID
Swiss Prot
Synonyms	ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; Phospho-EGFR-Y1068
Calculated MW	134kDa
Observed MW	185kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa treated by EGF or ATP
Cellular location	Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Customer validation	(Human non small cell lung adenocarcinoma cell) WB(Mus musculus, Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Phospho-EGFR-Y1068 Rabbit pAb images

Western blot - Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie

Application:

IF:

6.529

Species:

Mus musculus

PMID:

31055235

Title:

Gossypol overcomes EGFR-TKIs resistance in non-small cell lung cancer cells by targeting YAP/TAZ and EGFRL858R/T790M.

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Current cancer drug targets

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

30605061

Title:

Gefitinib represses JAK-STAT signaling activated by CRTC1-MAML2 fusion in mucoepidermoid carcinoma cells

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Homo sapiens

PMID:

35814257

Title:

LY2874455 and Abemaciclib Reverse FGF3/4/19/CCND1 Amplification Mediated Gefitinib Resistance in NSCLC - PMC

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

Homo sapiens

PMID:

36053436

Title:

Isobavachalcone's Alleviation of Pyroptosis Contributes to Enhanced Apoptosis in Glioblastoma: Possible Involvement of NLRP3

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Archives of biochemistry and biophysics

Application:

IF:

4.11

Species:

Homo sapiens

PMID:

37142078

Title:

Mechanisms of hedgehog, calcium and retinoic acid signalling pathway inhibitors: Plausible modes of action along the MLL–EZH2-p53 axis in cellular growth control

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Cancer letters

Application:

WB, IHC

IF:

9.756

Species:

Homo sapiens

PMID:

29981430

Title:

USP22 promotes resistance to EGFR-TKIs by preventing ubiquitination-mediated EGFR degradation in EGFR-mutant lung adenocarcinoma.

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Homo sapiens

PMID:

38253527

Title:

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC

References for Phospho-EGFR-Y1068 Rabbit pAb (AP0301)

Product:

Phospho-EGFR-Y1068 Rabbit pAb

Journal:

Journal of medicinal chemistry

Application:

IF:

7.3

Species:

Homo sapiens

PMID:

38471014