Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. 293T cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. C6 cells were treated by PMA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb |
|---|---|
| Catalog No. | AP0974 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1595 |
| Immunogen | A synthetic phosphorylated peptide around T202 & Y204 of human ERK1 (P27361). |
|---|---|
| Sequence | LTEYV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK; Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 |
| Calculated MW | 42kDa/44kDa |
| Observed MW | 42kDa/44kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | C6 treated by PMA, 293T treated by PMA |
| Cellular location | caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, focal adhesion, Golgi apparatus, late endosome, mitochondrion, nuclear envelope, nucleoplasm, nucleus, plasma membrane. |
| Customer validation | WB(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus, Other) IF(Mus musculus) Co-IP(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus) |
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