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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Publications (40) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. 293T cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. C6 cells were treated by PMA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Catalog No.AP0974
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1595
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic phosphorylated peptide around T202 & Y204 of human ERK1 (P27361).
SequenceLTEYV
Gene ID
Swiss Prot
SynonymsERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK; Phospho-ERK1-T202/Y204 + ERK2-T185/Y187
Calculated MW42kDa/44kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC6 treated by PMA, 293T treated by PMA
Cellular locationcaveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, focal adhesion, Golgi apparatus, late endosome, mitochondrion, nuclear envelope, nucleoplasm, nucleus, plasma membrane.
Customer validation

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus, Other)

IF(Mus musculus)

Co-IP(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)}

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

    Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. 293T cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)}

    Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

    Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (A4782) at1:3000 dilution (lower) incubated overnight at 4℃. C6 cells were treated by PMA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)}

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)}

    Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

    Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)}

    Immunofluorescence - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974)

    Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated), using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0974? Please let us know so that we can cite the reference in this datasheet.