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Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells, using Phospho-HP1 gamma/CBX3-S83 pAb (AP0801) at 1:1000 dilution or HP1 gamma/CBX3 antibody (A2248). HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Phospho-HP1 gamma/CBX3-S83 Rabbit pAb
Catalog No.	AP0801
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S83 of human HP1 gamma/CBX3 (NP_009207.2).
Sequence	RKSLS
Gene ID
Swiss Prot
Synonyms	HECH; HP1-GAMMA; HP1Hs-gamma; Phospho-HP1 gamma/CBX3-S83
Calculated MW	21kDa
Observed MW	22kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Paclitaxel, NIH/3T3 treated by Paclitaxel
Cellular location	Nucleus.