Reviews (2)Publications (38) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of lysates from 293T cells, using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Confocal imaging of 293T cells (treated with UV) and 293T cells (untreated) using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687, dilution 1:5000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells (treated with UV) and NIH/3T3 cells (untreated) using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687, dilution 1:5000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
| Product name | Phospho-Histone H2AX-S139 Rabbit mAb |
|---|---|
| Catalog No. | AP0687 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0110 |
| Immunogen | A synthetic phosphorylated peptide around S139 of human Histone H2A.X (P16104). |
|---|---|
| Sequence | TQASQ |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H2A.X; H2A/X; H2AFX; Phospho-Histone H2AX-S139 |
| Calculated MW | 15kDa |
| Observed MW | 17kDa/15kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | 293T treated by UV, NIH/3T3 treated by UV, C6 |
| Cellular location | Chromosome, Nucleus. |
| Customer validation | (SiHa cells, HUH7) IHC(Mus musculus, Homo sapiens) WB(Homo sapiens, Sus scrofa, Mus musculus) IF(Homo sapiens, Sus scrofa, Mus musculus, Macaca fascicularis, Drosophila melanogaster, Other, Bos taurus) IF(Homo sapiens, Mus musculus) WB(Drosophila melanogaster, Other) qPCR(Homo sapiens) |
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