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Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Reviews (3)Publications (63) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Phospho-Histone H2AX-S139 Rabbit pAb
ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of 293T(treated with UV) and 293T(untreated) cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)

Overview

Product namePhospho-Histone H2AX-S139 Rabbit pAb
Catalog No.AP0099
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
ImmunogenA synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
SequenceQASQEY
Gene ID
Swiss Prot
SynonymsH2A.X; H2A/X; H2AFX; Phospho-Histone H2AX-S139
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3 treated by UV, C6 treated by UV
Cellular locationChromosome, Nucleus.
Customer validation

(U251 cells, N2A, Mouse bone marrow)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens)

IF(Homo sapiens, Mus musculus)

ChIP(Arabidopsis thaliana)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at 1:400 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)}

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of 293T(treated with UV) and 293T(untreated) cells using Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (6)

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WBIHC-PIF/ICCELISA

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Phospho-Histone H2AX-S139 Rabbit mAb

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A2082

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Histone H2AX Rabbit pAb

Histone H2AX Rabbit pAb
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WBIHC-PIF/ICCELISA

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Histone H2AX Rabbit mAb
A11412

WBIHC-PELISAChIP

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Histone H2AX Rabbit mAb

PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb
AP1529

FC(intra)

Reactivity:Human

PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb

Phospho-Histone H2AX-S139 Rabbit mAb
AP1555

WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

Phospho-Histone H2AX-S139 Rabbit mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)