제품 > 항체 > 인산화 특이적 항체

Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-IκBα-S32/36 Rabbit pAb (AP0614) at 1:500 dilution. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-IκBα-S32/36 Rabbit pAb
Catalog No.	AP0614
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S32 & S36 of human IκBα (NP_065390.1).
Sequence	SGLDS
Gene ID
Swiss Prot
Synonyms	IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32/36
Calculated MW	36kDa
Observed MW	40kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by TNF-α and Calyculin A
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Cavia porcellus, Rattus norvegicus, Schisandra chinensis, Melodinus cochinchinensis, Mus musculus, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-IκBα-S32/36 Rabbit pAb images

Western blot - Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Inflammation research

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

28653218

Title:

2K1C-activated Angiotensin II (Ang II) exacerbates vascular damage in a rat model of arthritis through the ATR/ERK1/2 signaling pathway

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular and cell biology of lipids

Application:

IF:

3.9

Species:

Mus musculus

PMID:

30445165

Title:

Maternal eicosapentaenoic acid feeding promotes placental angiogenesis through a Sirtuin-1 independent inflammatory pathway.

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Fundamental & clinical pharmacology

Application:

IF:

2.9

Species:

Mus musculus

PMID:

31325387

Title:

Fraxin inhibits lipopolysaccharide-induced inflammatory cytokines and protects against endotoxic shock in mice.

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Cellular microbiology

Application:

IF:

3.4

Species:

Cavia porcellus

PMID:

31364251

Title:

Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-κB signalling pathway by binding to eEF1A1.

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Food & Function

Application:

IF:

5.1

Species:

Rattus norvegicus,Schisandra chinensis

PMID:

32186565

Title:

Investigation of the active components and mechanisms of Schisandra chinensis in the treatment of asthma based on a network pharmacology approach and experimental validation

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

International Immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

31784404

Title:

Anti-inflammatory effect of Yam Glycoprotein on lipopolysaccharide-induced acute lung injury via the NLRP3 and NF-κB/TLR4 signaling pathway

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

American Journal of Translational Research

Application:

IF:

1.7

Species:

Mus musculus

PMID:

27829992

Title:

HMGB1-TLR4 signaling participates in renal ischemia reperfusion injury and could be attenuated by dexamethasone-mediated inhibition of the ERK/NF-κB pathway.

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Molecules

Application:

IF:

4.6

Species:

Mus musculus

PMID:

31181689

Title:

Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway.

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

J Ethnopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

32590114

Title:

In vitro and in vivo anti-inflammatory effects of different extracts from Epigynum auritum through down-regulation of NF-κB and MAPK signaling pathways

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Cell Biochem Biophys

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

32705535

Title:

Hydroxysafflor Yellow A of Carthamus Tinctorius L., Represses the Malignant Development of Esophageal Cancer Cells via Regulating NF-κB Signaling Pathway

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.2

Species:

Homo sapiens

PMID:

32971102

Title:

PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escape

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Frontiers in Immunology

Application:

IF:

5.7

Species:

Mus musculus

PMID:

33324396

Title:

BAFF Blockade Attenuates Inflammatory Responses and Intestinal Barrier Dysfunction in a Murine Endotoxemia Model

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Tissue Cell

Application:

IF:

2.7

Species:

Mus musculus

PMID:

33676236

Title:

Protective effects of oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Exp Ther Med

Application:

IF:

2.4

Species:

Homo sapiens

PMID:

33884038

Title:

Role of Notch, IL-1 and leptin expression in colorectal cancer

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

6.7

Species:

Melodinus cochinchinensis

PMID:

34400050

Title:

Chemical constituents and anti-inflammatory activity of the total alkaloid extract from Melodinus cochinchinensis (Lour.) Merr. and its inhibition of the NF-κB and MAPK signaling pathways

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Environ Toxicol

Application:

IF:

4.5

Species:

Mus musculus

PMID:

34397165

Title:

Amelioratory effect of neoandrographolide on myocardial ischemic-reperfusion injury by its anti-inflammatory and anti-apoptotic activities

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

J Ethnopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

34038799

Title:

Optimization of ultrasound extraction of Tribulus terrestris L. leaves saponins and their HPLC-DAD-ESI-MSn profiling, anti-inflammatory activity and mechanism in vitro and in vivo

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.1

Species:

Mus musculus

PMID:

34057171

Title:

Preparation, characterization, and in vivo evaluation of anti-inflammatory activities of selenium nanoparticles synthesized by Kluyveromyces lactis GG799

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

J Ethnopharmacol

Application:

IF:

4.8

Species:

Mus musculus

PMID:

34116185

Title:

Anneslea fragrans Wall. ameliorates ulcerative colitis via inhibiting NF-κB and MAPK activation and mediating intestinal barrier integrity

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Evid Based Complement Alternat Med

Application:

IF:

Species:

Mus musculus

PMID:

34335857

Title:

Protective Effects of Anwulignan against HCl/Ethanol-Induced Acute Gastric Ulcer in Mice

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Eur J Pharmacol

Application:

IF:

4.2

Species:

Mus musculus

PMID:

34492285

Title:

Study on the effect of active components of Schisandra chinensis on liver injury and its mechanisms in mice based on network pharmacology

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

Species:

Homo sapiens

PMID:

34804360

Title:

Metformin Corrects Glucose Metabolism Reprogramming and NLRP3 Inflammasome-Induced Pyroptosis via Inhibiting the TLR4/NF-κB/PFKFB3 Signaling in Trophoblasts: Implication for a Potential Therapy of Preeclampsia

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.1

Species:

Homo sapiens, Mus musculus

PMID:

34108444

Title:

SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Biomed Pharmacother

Application:

IF:

6.9

Species:

Homo sapiens, Mus musculus

PMID:

34953394

Title:

Discovery of a novel cyclin-dependent kinase 8 inhibitor with an oxindole core for anti-inflammatory treatment

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Homo sapiens

PMID:

38398837

Title:

2-Hydroxychalcone Induces Autophagy and Apoptosis in Breast Cancer Cells via the Inhibition of the NF-B Signaling Pathway: In Vitro and In Vivo Studies - PMC

References for Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Product:

Phospho-IκBα-S32/36 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

30530164