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Phospho-IκBα-S32 Rabbit mAb (AP0707)

Review (1)Publications (84) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from C6 cells using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution (upper) or IκBα Rabbit mAb (A24909) at1:6000 dilution (lower) incubated overnight at 4℃. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Review
ABclonal: review for Phospho-IκBα-S32 Rabbit mAb(AP0707)

Overview

Product namePhospho-IκBα-S32 Rabbit mAb
Catalog No.AP0707
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0147
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenA synthetic phosphorylated peptide around S32 of human IKB alpha (P25963).
SequenceHDSGL
Gene ID
Swiss Prot
SynonymsIKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32
Calculated MW36kDa
Observed MW39kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by TNF-α, C6
Cellular locationCytoplasm, Nucleus.
Customer validation

(A549)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Felis catus, Gallus gallus, Mus musculus, Other, Sus scrofa)

IP(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)}

Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)}

Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from C6 cells using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution (upper) or IκBα Rabbit mAb (A24909) at1:6000 dilution (lower) incubated overnight at 4℃. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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