Review (1)Publications (10) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Phospho-IRS1-S307(Human Specific) Rabbit pAb (AP0552) at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-IRS1-S307(Human Specific) Rabbit pAb (AP0552) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Phospho-IRS1-S307(Human Specific) Rabbit pAb (AP0552) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Phospho-IRS1-S307(Human Specific) Rabbit pAb (AP0552) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
| Product name | Phospho-IRS1-S307(Human Specific) Rabbit pAb |
|---|---|
| Catalog No. | AP0552 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S307 of human IRS1 (NP_005535.1). |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HIRS-1; Phospho-IRS1-S307(Human Specific) |
| Calculated MW | 132kDa |
| Observed MW | 180kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | 293T treated by IGF-1 |
| Cellular location | caveola, cytoplasm, cytosol, nucleoplasm, nucleus, plasma membrane. |
| Customer validation | (mouse stomach cells) WB(Mus musculus, Rattus norvegicus, Homo sapiens) |
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