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Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (AP0777) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-Lamin A/C-S22 Rabbit pAb (AP0777)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Lamin A/C-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (AP0777) at a dilution of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.

Overview

Product name	Phospho-Lamin A/C-S22 Rabbit pAb
Catalog No.	AP0777
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S22 of human Lamin A (NP_005563.1).
Sequence	PLSPT
Gene ID
Swiss Prot
Synonyms	FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Phospho-Lamin A/C-S22
Calculated MW	74kDa
Observed MW	69kDa, 78kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa treated by Paclitaxel, C6 treated by Paclitaxel
Cellular location	Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation	WB(Rattus norvegicus)