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Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat heart using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb
Catalog No.	AP0396
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2?).
Sequence	PQTPL
Gene ID
Swiss Prot
Synonyms	MK2; MK-2; MAPKAP-K2; Phospho-MAPKAPK-2/MK2-T334
Calculated MW	46kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Immunohistochemistry
Positive samples
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Mus musculus)