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Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588)

Western blot analysis of various lysates using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-MAPKAPK-2/MK2-T334 Rabbit pAb
Catalog No.AP0588
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2).
SequencePQTPL
Gene ID
Swiss Prot
SynonymsMK2; MK-2; MAPKAP-K2; Phospho-MAPKAPK-2/MK2-T334
Calculated MW46kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by UV, C6 treated by PMA
Cellular locationCytoplasm, Nucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588)}

Western blot - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588)

Western blot analysis of various lysates using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0588) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (24)