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Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Publications (92) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Phospho-NF-kB p65/RelA-S276 Rabbit pAb
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at 1:400 dilution. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunofluorescence analysis of HeLa cells (treated with CA) and HeLa cells (untreated) using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-NF-kB p65/RelA-S276 Rabbit pAb
Catalog No.AP0123
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S276 of human RelA (NP_068810.3).
SequenceRPSDR
Gene ID
Swiss Prot
Synonymsp65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S276
Calculated MW60kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa treated by TNF-α and Calyculin A
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Rattus norvegicus, Mus musculus, Sus scrofa, Schisandra chinensis, Homo sapiens, Gallus gallus, Carassius gibelio, Mus musculus, Cyprinus carpio)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Mus musculus)

IF(Cyprinus carpio, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at 1:400 dilution. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)}

Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunofluorescence analysis of HeLa cells (treated with CA) and HeLa cells (untreated) using Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (15)

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Secondary Antibodies (25)