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Phospho-NRF2-S40 Rabbit mAb (AP1133)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-NRF2-S40 Rabbit mAb (A4859) at 1:1000 dilution.HepG2 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Phospho-NRF2-S40 Rabbit mAb (AP1133)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-NRF2-S40 Rabbit mAb (AP1133) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-NRF2-S40 Rabbit mAb (AP1133) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-NRF2-S40 Rabbit mAb
Catalog No.	AP1133
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0283

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S40 of human NRF2 (Q16236).
Sequence	DFSQR
Gene ID
Swiss Prot
Synonyms	NRF2; HEBP1; Nrf-2; IMDDHH; Phospho-NRF2-S40
Calculated MW	68kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HepG2
Cellular location	Cytoplasm, Nucleus, cytosol
Customer validation	WB(Mauremys sinensis , Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus) WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-NRF2-S40 Rabbit mAb images

Western blot - Phospho-NRF2-S40 Rabbit mAb (AP1133)

Immunohistochemistry - Phospho-NRF2-S40 Rabbit mAb (AP1133)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Aquat Toxicol

Application:

IF:

4.964

Species:

Mauremys sinensis

PMID:

33129562

Title:

Effects of ammonia exposure on antioxidant function, immune response and NF-κB pathway in Chinese Strip-necked Turtle (Mauremys sinensis)

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Mus musculus

PMID:

34634559

Title:

Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Evid Based Complement Alternat Med

Application:

IF:

2.629

Species:

Mus musculus

PMID:

34335857

Title:

Protective Effects of Anwulignan against HCl/Ethanol-Induced Acute Gastric Ulcer in Mice

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

The Journal of pharmacology and experimental therapeutics

Application:

IF:

4.4

Species:

Rattus norvegicus

PMID:

34131018

Title:

Anwulignan Ameliorates the Intestinal Ischemia/Reperfusion

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

9.98

Species:

Mus musculus

PMID:

36007796

Title:

Long-term exposure to PM2. 5 aggravates pulmonary fibrosis and acute lung injury by disrupting Nrf2-mediated antioxidant function

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

8.1

Species:

Mus musculus

PMID:

34890768

Title:

Inhibition of Nrf2 degradation alleviates age-related osteoporosis induced by 1, 25-Dihydroxyvitamin D deficiency

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

The Science of the total environment

Application:

IF:

10.75

Species:

Mus musculus

PMID:

36521597

Title:

Glyphosate-triggered hepatocyte ferroptosis via suppressing Nrf2/GSH/GPX4 axis exacerbates hepatotoxicity

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Rattus norvegicus

PMID:

36463827

Title:

Melatonin inhibits ferroptosis and delays age-related cataract by regulating SIRT6/p-Nrf2/GPX4 and SIRT6/NCOA4/FTH1 pathways

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Research square

Application:

IF:

Species:

Homo sapiens

PMID:

36778475

Title:

Regulation of Ferroptosis by Transcription Factor E2F1 and RB - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Mus musculus

PMID:

37373164

Title:

Empagliflozin Attenuates Vascular Calcification in Mice with Chronic Kidney Disease by Regulating the NFR2/HO-1 Anti-Inflammatory Pathway through AMPK Activation - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Homo sapiens

PMID:

37507910

Title:

Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Redox report : communications in free radical research

Application:

IHC, WB

IF:

5.69

Species:

Mus musculus

PMID:

38052218

Title:

Sulforaphane attenuates irradiation induced testis injury in mice

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

38320352

Title:

SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injury

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Aging

Application:

IF:

5.95

Species:

Mus musculus

PMID:

38295303

Title:

Treadmill exercise can regulate the redox balance in the livers of APP/PS1 mice and reduce LPS accumulation in their brains through the gut-liver-kupffer cell axis - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Homo sapiens, Mus musculus, Rattus norvegicus

PMID:

37531930

Title:

EP1 activation inhibits doxorubicin-cardiomyocyte ferroptosis via Nrf2 - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

Species:

Mus musculus

PMID:

38671893

Title:

Protective Effects of Isoliquiritigenin and Licochalcone B on the Immunotoxicity of BDE-47: Antioxidant Effects Based on the Activation of the Nrf2 Pathway and Inhibition of the NF-B Pathway - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Microorganisms

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38930573

Title:

Sulforaphane Inhibits Oxidative Stress and May Exert Anti-Pyroptotic Effects by Modulating NRF2/NLRP3 Signaling Pathway in Mycobacterium tuberculosis-Infected Macrophages - PMC

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

7.9

Species:

Homo sapiens,Mus musculus

PMID:

38878526

Title:

Hydroxysafflor yellow A ameliorates alcohol-induced liver injury through PI3K/Akt and STAT3/NF-B signaling pathways

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IHC

IF:

5.4

Species:

Mus musculus

PMID:

38750986

Title:

The protective effect of Lonicera japonica Thunb. against lipopolysaccharide-induced acute lung injury in mice: modulation of inflammation, oxidative stress, and

References for Phospho-NRF2-S40 Rabbit mAb (AP1133)

Product:

Phospho-NRF2-S40 Rabbit mAb

Journal:

Oncology reports

Application:

IF:

4.2

Species:

Homo sapiens

PMID:

38847277