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Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0477)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (AP0477) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0477)

Immunofluorescence analysis of C6 cells using Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0477) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0477) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Phospho-PDK1/PDPK1-S241 Rabbit pAb (AP0477) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-PDK1/PDPK1-S241 Rabbit pAb
Catalog No.	AP0477
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S241 of human PDK1/PDPK1 (NP_002604.1).
Sequence	ANSFV
Gene ID
Swiss Prot
Synonyms	PDK1; PDPK2; PDPK2P; PRO0461; Phospho-PDK1/PDPK1-S241
Calculated MW	63kDa
Observed MW	60-70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by PDGF
Cellular location	Cell junction, Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, focal adhesion.
Customer validation	WB(Mus musculus)