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Phospho-PEA15-S104 Rabbit pAb (AP0788)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa and C6 cells, using Phospho-PEA15-S104 Rabbit pAb (AP0788) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from HeLa cells, using Phospho-PEA15-S104 Rabbit pAb (A9956). HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	Phospho-PEA15-S104 Rabbit pAb
Catalog No.	AP0788
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
Sequence	IPSAK
Gene ID
Swiss Prot
Synonyms	PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15; Phospho-PEA15-S104
Calculated MW	15kDa
Observed MW	13kDa/15kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by PMA, C6 treated by PMA
Cellular location	Cytoplasm