Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from 293T cells, using Phospho-PERK-T982 Rabbit pAb (AP0886) at 1:1000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from A549 cells using Phospho-PERK-T982 Rabbit pAb (AP0886) at 1:1000 dilution incubated overnight at 4℃. A549 cells were treated by 10% FBS at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse Intestine tissue using Phospho-PERK-T982 Rabbit pAb (AP0886) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
| Product name | Phospho-PERK-T982 Rabbit pAb |
|---|---|
| Catalog No. | AP0886 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around T982 of human PERK (NP_004827.4). |
|---|---|
| Sequence | RHTGQ |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PEK; WRS; PERK; Phospho-PERK-T982 |
| Calculated MW | 125kDa |
| Observed MW | 170kDa/ |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | 293T treated by IGF-1, A549 treated by FBS |
| Cellular location | Endoplasmic reticulum membrane, Single-pass type I membrane protein. |
| Customer validation | WB(Mus musculus, Homo sapiens, Rattus norvegicus, Chlorocebus aethiops, Mus musculus, Danio rerio, Other, Anatinae) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP0886? Please let us know so that we can cite the reference in this datasheet.
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