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Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Publications (69) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis of lysates from wild type (WT) and 293T cells were transfected with SRC(Y530F), using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

Western blot analysis lysates from C2C12, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namePhospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb
Catalog No.AP0854
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
ImmunogenA synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1).
SequenceDRLYEE
Gene ID
Swiss Prot
SynonymsPIK3R1/PIK3R2/PIK3R3; Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464
Calculated MW42-54kDa/81-84kDa
Observed MW60kDa(P55)/85kDa(P85)
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with SRC(Y530F), C2C12 treated by Hydrogen Peroxide
Cellular locationcell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane.
Customer validation

WB(Rattus norvegicus, Homo sapiens, Schisandra chinensis, Mus musculus, Ctenopharyngodon idellus, Homo sapiens, Mus musculus, Gallus gallus, Other)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)}

    Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

    Western blot analysis of lysates from wild type (WT) and 293T cells were transfected with SRC(Y530F), using Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)}

    Western blot - Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854)

    Western blot analysis lysates from C2C12, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y199/Y464 Rabbit pAb (AP0854) at 1:1000 dilution.C2C12 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0854? Please let us know so that we can cite the reference in this datasheet.

    항체 (5)

    Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb
    AP0427

    WB

    Reactivity:Human, Mouse, Rat

    Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb

    PI3 Kinase p85 alpha/beta/gamma mAb
    A22996

    WBIF/ICCELISA

    Reactivity:Mouse, Rat

    PI3 Kinase p85 alpha/beta/gamma mAb

    ABflo® 488 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb
    AP1487

    FC(intra)

    Reactivity:Human

    ABflo® 488 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb

    ABflo® 647 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb
    AP1488

    FC(intra)

    Reactivity:Human

    ABflo® 647 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb

    ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb
    AP1489

    FC(intra)

    Reactivity:Human

    ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)