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Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (AP0191) at 1:1000 dilution or PRKCA antibody (A13342). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).

Overview

Product namePhospho-PKCalpha/beta II-T638/641 Rabbit pAb
Catalog No.AP0191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around T638 of human PKCalpha/beta IIPRKCA (NP_002728.2).
SequenceVLTPP
Gene ID
Swiss Prot
SynonymsPKC-beta; PKCB; PRKCB1; PRKCB2; Phospho-PKCalpha/beta II-T638/641
Calculated MW76kDa/77kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3 treated by PMA
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

    Western blot - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 pAb (AP0191) at 1:1000 dilution or PRKCA antibody (A13342). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

    Immunohistochemistry - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

    Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)}

    Immunofluorescence - Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191)

    Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PKCalpha/beta II-T638/641 Rabbit pAb (AP0191).

    * For research use only. Not for therapeutic or diagnostic purposes.

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