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Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from 293T cells, using Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749) at 1:1000 dilution or POLR2A antibody (A2107). 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-POLR2A CTD-S2 Rabbit pAb (AP0749)

Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg Phospho-POLR2A CTD-S2 antibody (AP0749). Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb (AP0749) at a dilution of 1:1000. MCF7 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.

Overview

Product name	Phospho-POLR2A CTD-S2 Rabbit pAb
Catalog No.	AP0749
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S2 of human POLR2A CTD repeat YSPTSPS.
Sequence	SYSPT
Gene ID
Swiss Prot
Synonyms	RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; Phospho-POLR2A CTD-S2
Calculated MW	217kDa
Observed MW	260kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	293T, 293T treated by Doxorubicin
Cellular location	Nucleus
Customer validation	WB(Homo sapiens, Mus musculus)