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Phospho-POLR2A CTD- S2/S5 Rabbit pAb (AP1335)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-POLR2A CTD- S2/S5 Rabbit pAb (AP1335)

Western blot analysis of HeLa, using Phospho-POLR2A CTD- S2/S5 antibody (AP1335) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

Overview

Product namePhospho-POLR2A CTD- S2/S5 Rabbit pAb
Catalog No.AP1335
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
ImmunogenA phospho specific peptide corresponding to residues surrounding S2/S5 of human POLR2A CTD repeat YSPTSPS.
SequenceSYSPT
Gene ID
Swiss Prot
SynonymsRPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220
Calculated MW217kDa
Observed MW250kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationNucleus

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ABclonal:Western blot - Phospho-POLR2A CTD- S2/S5 Rabbit pAb (AP1335)}

Western blot - Phospho-POLR2A CTD- S2/S5 Rabbit pAb (AP1335)

Western blot analysis of HeLa, using Phospho-POLR2A CTD- S2/S5 antibody (AP1335) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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