Western blot - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37℃ for 24 hours or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)
Immunofluorescence analysis of NIH/3T3 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.