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Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37℃ for 24 hours or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-POLR2A CTD-S5 Rabbit mAb
Catalog No.AP0997
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1541
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
ImmunogenA phospho specific peptide corresponding to residues surrounding S5 of human POLR2A CTD (P24928).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsRPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; Phospho-POLR2A CTD-S5
Calculated MW217kDa
Observed MW270kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7 treated by Doxorubicin, MCF7 treated by nocodazole, C2C12
Cellular locationNucleus.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Western blot - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37℃ for 24 hours or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunohistochemistry - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)}

Immunofluorescence - Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-POLR2A CTD-S5 Rabbit mAb (AP0997) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (11)

Secondary Antibodies (26)