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Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from MCF7 cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37℃ for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Chromatin Immunoprecipitation - Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969)

Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using Phospho-POLR2A CTD-S7 Rabbit pAb (AP0969) and rabbit IgG (AC005). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.

Overview

Product name	Phospho-POLR2A CTD-S7 Rabbit pAb
Catalog No.	AP0969
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S7 of human POLR2A CTD repeat YSPTSPS.
Sequence	SPSYS
Gene ID
Swiss Prot
Synonyms	RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; Phospho-POLR2A CTD-S7
Calculated MW	217kDa
Observed MW	270kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	MCF7 treated by Doxorubicin, MCF7 treated by nocodazole
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens) IHC(Homo sapiens)