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Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325) at1:6000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Western blot analysis of lysates from PC-12 cells, using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325) at1:6000 dilution. PC-12 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s

ABclonal:Immunohistochemistry - Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325) at dilution of 1:500 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb (AP1325) at dilution of 1:500 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb
Catalog No.	AP1325
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53843

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S235 & S236 of human S6 Ribosomal Protein (RPS6) (NP_001001.2).
Sequence	RLSSLR
Gene ID
Swiss Prot
Synonyms	S6; eS6; Phospho-S6 Ribosomal Protein (RPS6)-S235/236
Calculated MW	29kDa
Observed MW	32kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:7000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	NIH/3T3, PC-12 treated by FBS
Cellular location	cytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm.