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Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537) at 1:1000 dilution or RPS6 antibody (A6058). HeLa and NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb (AP0537)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg Phospho-S6 Ribosomal Protein (RPS6)-S240/244 pAb (AP0537). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 pAb (AP0537) at a dilution of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.

Overview

Product name	Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit pAb
Catalog No.	AP0537
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2).
Sequence	STSKS
Gene ID
Swiss Prot
Synonyms	S6; eS6; Phospho-S6 Ribosomal Protein (RPS6)-S240/244
Calculated MW	29kDa
Observed MW	36kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa treated by 10% FBS, NIH/3T3 treated by 10% FBS
Cellular location	cytoplasm, cytoplasmic ribonucleoprotein granule, cytosol, cytosolic ribosome, endoplasmic reticulum, nucleolus, nucleoplasm, nucleus, perinuclear region of cytoplasm.
Customer validation	WB(Homo sapiens, Mus musculus) IF(Danio rerio, Other)