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Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-STAT1-Y701 Rabbit mAb (AP0054)at 1:1000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and PC-12 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product name	Phospho-STAT1-Y701 Rabbit mAb
Catalog No.	AP0054
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0049

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y701 of human STAT1 (P42224).
Sequence	TGYIK
Gene ID
Swiss Prot
Synonyms	CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; Phospho-STAT1-Y701
Calculated MW	87kDa
Observed MW	84, 91kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, NIH/3T3, PC-12
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Mus musculus) IF(Homo sapiens, Mus musculus) IF(Homo sapiens) WB(Homo sapiens) qPCR(Mus musculus) IHC(Mus musculus)

Documents

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Research Area

Phospho-STAT1-Y701 Rabbit mAb images

Western blot - Phospho-STAT1-Y701 Rabbit mAb (AP0054)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Molecular biomedicine

Application:

IF:

Species:

Homo sapiens

PMID:

35945406

Title:

MicroRNA-379-5p regulates free cholesterol accumulation and relieves diet induced-liver damage in db/db mice via STAT1/HMGCS1 axis - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Homo sapiens

PMID:

36364345

Title:

Polygalaxanthone III, an Active Ingredient in Polygala japonica Houtt., Repaired Malassezia-Stimulated Skin Injury via STAT3 Phosphorylated Activation - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Mus musculus

PMID:

36429017

Title:

Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB Pathway - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Biochemical pharmacology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

36563885

Title:

Exploring the potential molecular mechanism of trastuzumab-induced cardiotoxicity based on RNA sequencing and bioinformatics analysis

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Archives of oral biology

Application:

IF:

2.64

Species:

Rattus norvegicus

PMID:

36410162

Title:

Parasympathetic-macrophages-ductal epithelial cells axis promotes female rat submandibular gland regeneration after excretory duct ligation/deligation

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

36739832

Title:

Upadacitinib inhibits corneal inflammation and neovascularization by suppressing M1 macrophage infiltration in the corneal alkali burn model

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

36739636

Title:

Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

mBio

Application:

IF:

Species:

Homo sapiens

PMID:

36786573

Title:

Strategy To Assess Zoonotic Potential Reveals Low Risk Posed by SARS-Related Coronaviruses from Bat and Pangolin

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Homo sapiens

PMID:

37744350

Title:

TAGAP expression influences CD4+ T cell differentiation, immune infiltration, and cytotoxicity in LUAD through the STAT pathway: implications for immunotherapy - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Inflammation

Application:

IF:

4.65

Species:

Mus musculus

PMID:

37679586

Title:

NMI Functions as Immuno-regulatory Molecule in Sepsis by Regulating Multiple Signaling Pathways

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Virus research

Application:

IF:

6.28

Species:

Sus scrofa

PMID:

38092254

Title:

Angiotensin converting enzyme 2 does not facilitate porcine epidemic diarrhea virus entry into porcine intestinal epithelial cells and inhibits it-induced inflammatory injury by promoting STAT1 phosphorylation - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

WB, IF

IF:

7.52

Species:

Homo sapiens, Mus musculus

PMID:

38110975

Title:

GETV nsP2 plays a critical role in the interferon antagonism and viral pathogenesis - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Regenerative therapy

Application:

IF:

3.65

Species:

Homo sapiens

PMID:

38327716

Title:

Extracellular vesicles derived from HuMSCs alleviate daunorubicin-induced cardiac microvascular injury via miR-186-5p/PARP9/STAT1 signal pathway - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

37979167

Title:

E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

PLoS pathogens

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38377149

Title:

The NP protein of Newcastle disease virus dictates its oncolytic activity by regulating viral mRNA translation efficiency - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Frontiers in cell and developmental biology

Application:

IF:

6.08

Species:

Mus musculus

PMID:

35111758

Title:

Harmine Alleviated Sepsis-Induced Cardiac Dysfunction by Modulating Macrophage Polarization via the STAT/MAPK/NF-B Pathway.

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Virus research

Application:

IF:

6.28

Species:

Sus scrofa

PMID:

37995963

Title:

Npro of classical swine fever virus enhances HMGB1 acetylation and its degradation by lysosomes to evade from HMGB1-mediated antiviral immunity - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF, WB

IF:

8.2

Species:

Homo sapiens

PMID:

38458291

Title:

Porcine deltacoronavirus NS7a antagonizes JAK/STAT pathway by inhibiting the interferon-stimulated gene factor 3 (ISGF3) formation

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Translational vision science & technology

Application:

IF:

3.048

Species:

Homo sapiens,Rattus norvegicus

PMID:

35353151

Title:

Topical Administration of 0.3% Tofacitinib Suppresses M1 Macrophage Polarization and Allograft Corneal Rejection by Blocking STAT1 Activation in the Rat Cornea

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Journal for immunotherapy of cancer

Application:

IF:

10.9

Species:

Homo sapiens

PMID:

38749537

Title:

Original research: Colorectal cancer-specific IFN delivery overcomes dysfunctional dsRNA-mediated type I interferon signaling to increase the abscopal effect of radiotherapy - PMC

References for Phospho-STAT1-Y701 Rabbit mAb (AP0054)

Product:

Phospho-STAT1-Y701 Rabbit mAb

Journal:

Journal of Cancer

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

38817865