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Phospho-STAT3-S727 Rabbit mAb (AP0715)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (AP0715) at 1:1000 dilution. NIH/3T3 and PC-12 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit mAb (AP0715)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-STAT3-S727 Rabbit mAb (AP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review

Overview

Product name	Phospho-STAT3-S727 Rabbit mAb
Catalog No.	AP0715
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0150

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S727 of human Phospho-Phospho-STAT3-S727 (S727) (P40763).
Sequence	PMSPR
Gene ID
Swiss Prot
Synonyms	APRF; Phospho-STAT3-S727
Calculated MW	88kDa
Observed MW	88kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	PC-12 treated by TNF-α, NIH/3T3 treated by TNF-α
Cellular location	Cytoplasm, Nucleus
Customer validation	(HeLa(Starvation)、HeLa(FBS)、HeLa(EGF)、HeLa(IGF)、HeLa(Insulin)、293T(Starvation)、293T(FBS)、293T(EGF)、293T(IGF)、) IHC(Mus musculus) WB(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus) IF(Homo sapiens, Rattus norvegicus) IHC(Rattus norvegicus) Co-IP(Rattus norvegicus) IF(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-STAT3-S727 Rabbit mAb images

Western blot - Phospho-STAT3-S727 Rabbit mAb (AP0715)

Immunohistochemistry - Phospho-STAT3-S727 Rabbit mAb (AP0715)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Frontiers in oncology

Application:

IHC

IF:

5.73

Species:

Mus musculus

PMID:

35936759

Title:

5-Methoxytryptophan Sensitizing Head and Neck Squamous Carcinoma Cell to Cisplatitn Through Inhibiting Signal Transducer and Activator of Transcription 3 (STAT3) - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Homo sapiens

PMID:

36364345

Title:

Polygalaxanthone III, an Active Ingredient in Polygala japonica Houtt., Repaired Malassezia-Stimulated Skin Injury via STAT3 Phosphorylated Activation - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Annals of medicine and surgery (2012)

Application:

IF:

Species:

Mus musculus

PMID:

37915641

Title:

Xiaoqinglong decoction suppresses childhood cough variant asthma and inhibited the body inflammatory response by regulating IL-6/STAT3 signalling pathway - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

37960182

Title:

Protein Disulfide Isomerase Family A Member 3 Knockout Abrogate Effects of Vitamin D on Cellular Respiration and Glycolysis in Squamous Cell Carcinoma - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

37894738

Title:

Notopterol Suppresses IL-17-Induced Proliferation and Invasion of A549 Lung Adenocarcinoma Cells via Modulation of STAT3, NF-B, and AP-1 Activation - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Frontiers in psychiatry

Application:

IF:

5.435

Species:

Homo sapiens, Mus musculus

PMID:

35509887

Title:

Deciphering Risperidone-Induced Lipogenesis by Network Pharmacology and Molecular Validation

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Drug development research

Application:

IF:

Species:

Homo sapiens

PMID:

36852868

Title:

Kaempferol suppresses glioma progression and synergistically enhances the antitumor activity of gefitinib by inhibiting the EGFR/SRC/STAT3 signaling pathway

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Chemosphere

Application:

IF:

8.8

Species:

Homo sapiens

PMID:

37742764

Title:

Zinc attenuates sulfamethoxazole-induced lipotoxicity by reversing sulfamethoxazole-induced mitochondrial dysfunction and lysosome impairment in a freshwater teleost

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

BMC genomics

Application:

IF:

4.54

Species:

Mus musculus

PMID:

38310234

Title:

Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

WB, IF

IF:

5.21

Species:

Homo sapiens

PMID:

36678513

Title:

Preclinical Studies of Chiauranib Show It Inhibits Transformed Follicular Lymphoma through the VEGFR2/ERK/STAT3 Signaling Pathway - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

38043267

Title:

Upregulation of PTPN1 aggravates endotoxemia-induced cardiac dysfunction through inhibiting mitophagy

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Research (Washington, D.C.)

Application:

IF:

Species:

Homo sapiens

PMID:

38420580

Title:

Gradient Rotating Magnetic Fields Impairing F-Actin-Related Gene CCDC150 to Inhibit Triple-Negative Breast Cancer Metastasis by Inactivating TGF-1/SMAD3 Signaling Pathway - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens, Rattus norvegicus

PMID:

36613500

Title:

Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Life sciences

Application:

WB, IHC

IF:

6.78

Species:

Rattus norvegicus

PMID:

36804309

Title:

Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

WB, IF, Co-IP

IF:

5.19

Species:

Rattus norvegicus

PMID:

37120058

Title:

The alcohol extracts of Sceptridium ternatum (Thunb.) Lyon exert anti-pulmonary fibrosis effect through targeting SETDB1/STAT3/p-STAT3 signaling

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Journal of cachexia, sarcopenia and muscle

Application:

IF:

8.9

Species:

Mus musculus

PMID:

38553831

Title:

The regulation of MFGE8 on the mitophagy in diabetic sarcopenia via the HSPA1LParkin pathway and the effect of Dpinitol

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Immunity, inflammation and disease

Application:

IF:

3.2

Species:

Rattus norvegicus

PMID:

38896093

Title:

Modified Hongteng Baijiang decoction enema improves sequelae of pelvic inflammatory disease by regulating the LIF/JAK2/STAT3 pathway and gut microbiota - PMC

References for Phospho-STAT3-S727 Rabbit mAb (AP0715)

Product:

Phospho-STAT3-S727 Rabbit mAb

Journal:

Cancer letters

Application:

IF, WB

IF:

9.7

Species:

Homo sapiens,Mus musculus

PMID: