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Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Review (1)Publications (36) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Confocal imaging of paraffin-embedded Mouse colon tissue using Phospho-STAT3-Y705 Rabbit mAb (AP0705, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Review
ABclonal: review for Phospho-STAT3-Y705 Rabbit mAb(AP0705)

Overview

Product namePhospho-STAT3-Y705 Rabbit mAb
Catalog No.AP0705
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50831
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
ImmunogenA synthetic phosphorylated peptide around Y705 of human STAT3 (NP_644805.1).
SequenceAPYLK
Gene ID
Swiss Prot
SynonymsAPRF; HIES; ADMIO; ADMIO1; Phospho-STAT3-Y705
Calculated MW88kDa
Observed MW79kDa/88kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3 treated with TNF-α
Cellular locationCytoplasm, Nucleus.
Customer validation

(293T 、293T(饥饿)、293T(FBS) 、Hela 、Hela(饥饿)、Hela(insulin)))

WB(Homo sapiens, Mus musculus, Mus musculus, Horabagrus nigricollaris, Rattus norvegicus)

IP(Homo sapiens)

IF(Homo sapiens, Mus musculus)

IHC(Mus musculus, Homo sapiens)

WB(Mus musculus, Homo sapiens)

RT-qPCR(Homo sapiens)

IF(Homo sapiens)

Documents

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ABclonal:Western blot - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Western blot - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunohistochemistry - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AP0705) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-STAT3-Y705 Rabbit mAb (AP0705)}

Immunofluorescence - Phospho-STAT3-Y705 Rabbit mAb (AP0705)

Confocal imaging of paraffin-embedded Mouse colon tissue using Phospho-STAT3-Y705 Rabbit mAb (AP0705, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (8)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)