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Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Publications (51) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at 1:800 dilution. C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb
Catalog No.AP0548
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
ImmunogenA synthetic phosphorylated peptide around S465 & S467 of human Phospho-Smad2-S465/467 + Smad3-S423/425SMAD2 (NP_005892.1).
SequenceRCSSMS
Gene ID
Swiss Prot
SynonymsHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; Phospho-Smad2-S465/467 + Smad3-S423/425
Calculated MW48kDa/52kDa/25kDa/35kDa/43kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC2C12 treated by TGF-β
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus, Other)

IF(Homo sapiens, Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

IF(Gallus gallus, Rattus norvegicus)

ChIP(Rattus norvegicus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Western blot - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Western blot analysis of lysates from C2C12 cells, using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at 1:800 dilution. C2C12 cells were treated by TGF-β (10 ng/ml) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunohistochemistry analysis of paraffin-embedded Mouse stomach using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunohistochemistry - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunofluorescence analysis of L929 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunofluorescence analysis of PC-12 cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)}

    Immunofluorescence - Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548)

    Immunofluorescence analysis of U2OS cells using Phospho-Smad2-S465/467 + Smad3-S423/425 Rabbit pAb (AP0548) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0548? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb
    AP1343

    WBELISA

    Reactivity:Human, Mouse, Rat

    Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb

    Secondary Antibodies (25)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb