Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-Smad3-S423/S425 Rabbit mAb (AP0727) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | Phospho-Smad3-S423/S425 Rabbit mAb |
|---|---|
| Catalog No. | AP0727 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0170 |
| Immunogen | A synthetic phosphorylated peptide around S423 & S425 of human Smad3 (P84022). |
|---|---|
| Sequence | SSVS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; Phospho-Smad3-S423/S425 |
| Calculated MW | 48kDa |
| Observed MW | 55kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | C6 treated by PMA |
| Cellular location | Cytoplasm, Nucleus. |
| Customer validation | WB(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus) IHC(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Homo sapiens, Mus musculus) IF(Homo sapiens, Mus musculus) WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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