제품 > 항체 > 인산화 특이적 항체

Phospho-Syk-Y525/526 Rabbit pAb (AP0542)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Syk-Y525/526 Rabbit pAb (AP0542)

Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (AP0542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-Syk-Y525/526 Rabbit pAb
Catalog No.AP0542
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y525 & Y526 of human Syk (NP_003168.2).
SequenceENYYKA
Gene ID
Swiss Prot
SynonymsIMD82; p72-Syk; Phospho-Syk-Y525/526
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat
Cellular locationCell membrane, Cytoplasm, cytosol
Customer validation

WB(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - Phospho-Syk-Y525/526 Rabbit pAb (AP0542)}

Western blot - Phospho-Syk-Y525/526 Rabbit pAb (AP0542)

Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (AP0542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0542? Please let us know so that we can cite the reference in this datasheet.

항체 (7)

Secondary Antibodies (26)