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Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from A-431 cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-VEGF Receptor 2-Y1175 Rabbit pAb
Catalog No.	AP0382
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2 (NP_002244.1).
Sequence	KDYIV
Gene ID
Swiss Prot
Synonyms	FLK1; CD309; VEGFR; VEGFR2; Phospho-VEGF Receptor 2-Y1175
Calculated MW	152kDa
Observed MW	230kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431 treated by EGF
Cellular location	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Early endosome, Endoplasmic reticulum, Nucleus, Secreted, Single-pass type I membrane protein.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus) IHC(Homo sapiens) IF(Homo sapiens, Gallus gallus) IF(Rattus norvegicus) RT-PCR(Mus musculus)

Documents

Certificate of Compliance

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Research Area

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb images

Western blot - Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Homo sapiens

PMID:

27966526

Title:

Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Bone

Application:

IF:

4.398

Species:

Mus musculus

PMID:

30610968

Title:

Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis.

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Cell Metabolism

Application:

IF:

27.287

Species:

Mus musculus

PMID:

32375062

Title:

Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

J Cancer

Application:

IF:

4.207

Species:

Homo sapiens

PMID:

34659545

Title:

P68 RNA Helicase facilitates Breast Cancer progression by promoting Proliferation and Migration via PDGFR-β/AR axis

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Translational oncology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

36054996

Title:

Comprehensive analysis to identify the RP11–478C19.2/ E2F7 axis as a novel biomarker for treatment decisions in clear cell renal cell carcinoma - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

37716496

Title:

Txnrd3 knockout enhancement of lung injury induced by Ni exposure via the VEGF-VEGFR-2 axis and alleviation of this effect by melatonin

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IHC, IF

IF:

6.2

Species:

Homo sapiens

PMID:

36982218

Title:

HSPA5 Promotes the Proliferation, Metastasis and Regulates Ferroptosis of Bladder Cancer - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

WB, IF

IF:

5.21

Species:

Homo sapiens

PMID:

36678513

Title:

Preclinical Studies of Chiauranib Show It Inhibits Transformed Follicular Lymphoma through the VEGFR2/ERK/STAT3 Signaling Pathway - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Regenerative therapy

Application:

WB, IF

IF:

3.65

Species:

Rattus norvegicus

PMID:

38178930

Title:

Bi-functional KIT-PR1P peptides combine with VEGF to protect ischemic kidney in rats by targeting to Kim-1 - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Combinatorial Chemistry & High Throughput Screening

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

36852790

Title:

Apatinib Inhibits Bladder Cancer through Suppression of the VEGFR2- PI3K-AKT Signaling Pathway as Revealed by Network Pharmacology and in vitro Experimental Verification

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

38158116

Title:

Cediranib ameliorates portal hypertensive syndrome via inhibition of VEGFR-2 signaling in cirrhotic rats

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Poultry science

Application:

WB, IF

IF:

4.01

Species:

Gallus gallus

PMID:

36621100

Title:

Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1-F3) in late-phase laying hens

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.01

Species:

Gallus gallus

PMID:

35679671

Title:

Promotion Effect of Angelica Sinensis Extract on Angiogenesis of Chicken Preovulatory Follicles in Vitro

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Poultry science

Application:

WB, IF

IF:

4.01

Species:

Gallus gallus

PMID:

36566660

Title:

Effect of Angelica Sinensis extract on the angiogenesis of preovulatory follicles (F1-F3) in late-phase laying hens - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

WB, RT-PCR

IF:

6.37

Species:

Mus musculus

PMID:

37936054

Title:

PP121, a dual inhibitor of tyrosine and phosphoinositide kinases, relieves airway hyperresponsiveness, mucus hypersecretion and inflammation in a murine asthma model - PMC

References for Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (AP0382)

Product:

Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Journal:

Journal of translational medicine

Application:

IF:

7.4

Species:

Homo sapiens

PMID:

38702818