제품 > 항체 > 인산화 특이적 항체

Phospho-YAP1-S127 Rabbit mAb (AP1436)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-YAP1-S127 Rabbit mAb (AP1436) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from C6 cells, using Phospho-YAP1-S127 Rabbit mAb (AP1436) at 1:1000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Phospho-YAP1-S127 Rabbit mAb (AP1436)

Immunofluorescence analysis of puromycin in HeLa cells (treated with CA) and HeLa cells (untreated) using Phospho-YAP1-S127 Rabbit mAb (AP1436) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of puromycin in NIH/3T3 cells (treated with CA) and HeLa cells (untreated) using Phospho-YAP1-S127 Rabbit mAb (AP1436) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of puromycin in C6 cells (treated with CA) and HeLa cells (untreated) using Phospho-YAP1-S127 Rabbit mAb (AP1436) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-YAP1-S127 Rabbit mAb
Catalog No.	AP1436
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61727

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S127 of human YAP1(NP_001123617.1).
Sequence	RAHSSP
Gene ID
Swiss Prot
Synonyms	YAP1; COB1; YAP; YAP2; YAP65; YKI; Yes associated protein 1; Phospho-YAP1-S127
Calculated MW	36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW	75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa treated by Calyculin A, C6 treated by Calyculin A
Cellular location	Cytoplasm, Nucleus.