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Phospho-eIF2α-S49 Rabbit pAb (AP0341)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-eIF2α-S49 Rabbit pAb (AP0341).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunohistochemistry - Phospho-eIF2α-S49 Rabbit pAb (AP0341)

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-eIF2α-S49 Rabbit pAb (AP0341).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-eIF2α-S49 Rabbit pAb
Catalog No.	AP0341
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S49 of human EIF2S1
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; Phospho-eIF2α-S49
Calculated MW	36kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa
Cellular location	Cytoplasmic granule
Customer validation	WB(Mus musculus, Homo sapiens) IF(Mus musculus)