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Phospho-eNOS-S1177 Rabbit mAb (AP1404)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from 293T lysates cells, using Phospho-eNOS-S1177 Rabbit mAb (AP1404) at 1:7000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Phospho-eNOS-S1177 Rabbit mAb
Catalog No.	AP1404
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58017

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Sequence	TQSFS
Gene ID
Swiss Prot
Synonyms	eNOS; ECNOS; Phospho-eNOS-S1177
Calculated MW	133kDa
Observed MW	150kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:8000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by IGF-1
Cellular location	Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Caveola, Cytoskeleton
Customer validation	WB(Mus musculus)