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Phospho-eNOS-S1177 Rabbit pAb (AP0421)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (AP0421). Jurkat cells were treated by Calyculin A.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit pAb (A1548). HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product name	Phospho-eNOS-S1177 Rabbit pAb
Catalog No.	AP0421
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Sequence	TQSFS
Gene ID
Swiss Prot
Synonyms	eNOS; ECNOS; Phospho-eNOS-S1177
Calculated MW	133kDa
Observed MW	133kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat treated by Calyculin A, HeLa treated by IGF-1
Cellular location	Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton.
Customer validation	WB(Homo sapiens, Mus musculus ) IHC(Rattus norvegicus)