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Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (AP0115) at 1:1000 dilution. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-mTOR-S2448 Rabbit mAb (AP0115) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	Phospho-mTOR-S2448 Rabbit mAb
Catalog No.	AP0115
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0094

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S2448 of human mTOR (P42345).
Sequence	TDSYS
Gene ID
Swiss Prot
Synonyms	SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Phospho-mTOR-S2448
Calculated MW	289kDa
Observed MW	289kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by 10% FBS, 293T treated by IGF-1, NIH/3T3 treated by EGF
Cellular location	Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein.
Customer validation	IHC(Mus musculus) WB(Spodoptera frugiperda, Homo sapiens, Mus musculus, Gallus gallus, Rattus norvegicus, Oryctolagus cuniculus) IF(Homo sapiens) FC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-mTOR-S2448 Rabbit mAb images

Western blot - Phospho-mTOR-S2448 Rabbit mAb (AP0115)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cell Metabolism

Application:

IHC

IF:

27.287

Species:

Mus musculus

PMID:

32375062

Title:

Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Virulence

Application:

IF:

5.882

Species:

Spodoptera frugiperda

PMID:

33509041

Title:

Autophagy induced by Vip3Aa has a pro-survival role in Spodoptera frugiperda Sf9 cells

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

32508661

Title:

Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Pharmaceuticals (Basel)

Application:

IF:

Species:

Homo sapiens

PMID:

33800129

Title:

Carnosic Acid Induces Apoptosis and Inhibits Akt/mTOR Signaling in Human Gastric Cancer Cell Lines

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

In Vitro Cell Dev Biol Anim

Application:

IF:

2.416

Species:

Homo sapiens

PMID:

34888748

Title:

Nucleoporin 37 promotes the cell proliferation, migration, and invasion of gastric cancer through activating the PI3K/AKT/mTOR signaling pathway

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of clinical medicine

Application:

IF:

4.96

Species:

Homo sapiens

PMID:

35806945

Title:

Morusin Enhances Temozolomide Efficiency in GBM by Inducing Cytoplasmic Vacuolization and Endoplasmic Reticulum Stress - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Mediators of inflammation

Application:

IF:

4.52

Species:

Mus musculus

PMID:

36133744

Title:

PI3K/AKT/mTOR Signaling Pathway Is Downregulated by Runzaoling (RZL) in Sjögren's Syndrome - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

9.68

Species:

Homo sapiens

PMID:

35725558

Title:

Co-loaded lapatinib/PAB by ferritin nanoparticles eliminated ECM-detached cluster cells via modulating EGFR in triple-negative breast cancer - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36430694

Title:

Mallotucin D, a Clerodane Diterpenoid from Croton crassifolius, Suppresses HepG2 Cell Growth via Inducing Autophagic Cell Death and Pyroptosis - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Poultry science

Application:

IF:

4.01

Species:

Gallus gallus

PMID:

36525749

Title:

Folate inhibits lipid deposition via the autophagy pathway in chicken hepatocytes - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of endocrinological investigation

Application:

IF:

5.46

Species:

Rattus norvegicus

PMID:

36427136

Title:

MicroRNA-137 inhibits pituitary prolactinoma proliferation by targeting AKT2

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

36842723

Title:

Hydroxysafflor yellow a confers neuroprotection against acute traumatic brain injury by modulating neuronal autophagy to inhibit NLRP3 inflammasomes

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Mus musculus

PMID:

36563293

Title:

Novel Pro-myogenic Factor Neoruscogenin Induces Muscle Fiber Hypertrophy by Inhibiting MSTN Maturation and Activating the Akt/mTOR Pathway

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Thoracic cancer

Application:

IF:

3.22

Species:

Homo sapiens

PMID:

36919755

Title:

UHMK1 promotes lung adenocarcinoma oncogenesis by regulating the PI3K/AKT/mTOR signaling pathway

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

International journal of molecular medicine

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

37026504

Title:

Dihydroartemisinin inhibits the activation and proliferation of hepatic stellate cells by regulating miR-29b-3p - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Theriogenology

Application:

IF:

2.92

Species:

Gallus gallus

PMID:

37879287

Title:

miRNA-29-3p targets PTEN to regulate follicular development through the PI3K/Akt/mTOR signaling pathway

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

4.81

Species:

Homo sapiens

PMID:

37934902

Title:

Maackiain suppresses the development of cervical cancer via AMPK priming autophagy

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Naunyn-Schmiedeberg's archives of pharmacology

Application:

IF:

3.19

Species:

Homo sapiens

PMID:

37676495

Title:

Autophagy inhibition mediated by trillin promotes apoptosis in hepatocellular carcinoma cells via activation of mTOR/STAT3 signaling

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of Translational Medicine

Application:

IF:

7.4

Species:

Homo sapiens

PMID:

37633909

Title:

Glutathione Peroxidase 3 induced mitochondria-mediated apoptosis via AMPK /ERK1/2 pathway and resisted autophagy-related ferroptosis via AMPK/mTOR pathway in hyperplastic prostate

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cellular Signalling

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

36473621

Title:

DHCR7 promotes tumorigenesis via activating PI3K/AKT/mTOR signalling pathway in bladder cancer

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

35795282

Title:

Isoschaftoside Reverses Nonalcoholic Fatty Liver Disease via Activating Autophagy In Vivo and In Vitro - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cardiovascular therapeutics

Application:

IF:

3.36

Species:

Homo sapiens

PMID:

38125704

Title:

Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of molecular histology

Application:

WB, IF

IF:

3.15

Species:

Homo sapiens

PMID:

36002678

Title:

mTOR is involved in LRP5-induced osteogenic differentiation of normal and aged periodontal ligament stem cells in vitro

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Homo sapiens

PMID:

38353382

Title:

Galectin-9 alleviates acute graft-versus-host disease after haplo-hematopoietic stem cell transplantation by regulating regulatory T cell/effector T cell imbalance - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Rattus norvegicus

PMID:

37338809

Title:

L-theanine attenuates heat stress-induced proteotoxicity and alterations in carbohydrate and lipid metabolism via heat shock factor 1

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

8.9

Species:

Homo sapiens

PMID:

37967710

Title:

Environment relevant exposure of perfluorooctanoic acid accelerates the growth of hepatocellular carcinoma cells through mammalian target of rapamycin (mTOR)

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

38413708

Title:

Isocitrate dehydrogenase 2 regulates the proliferation of triple-negative breast cancer through the ferroptosis pathway - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of orthopaedic surgery and research

Application:

IF:

2.6

Species:

Homo sapiens

PMID:

38468339

Title:

Oxymatrine protects articular chondrocytes from IL-1-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Gene

Application:

IF:

3.91

Species:

Homo sapiens

PMID:

37257791

Title:

TLR4 inhibited autophagy by modulating PI3K/AKT/mTOR signaling pathway in Gastric cancer cell lines

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cancers

Application:

IF:

6.57

Species:

Homo sapiens

PMID:

36428630

Title:

UCHL5 Promotes Proliferation and Migration of Bladder Cancer Cells by Activating c-Myc via AKT/mTOR Signaling - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Homo sapiens

PMID:

36429034

Title:

LTB4R Promotes the Occurrence and Progression of Clear Cell Renal Cell Carcinoma (ccRCC) by Regulating the AKT/mTOR Signaling Pathway - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Homo sapiens

PMID:

37269775

Title:

Sophflarine A, a novel matrine-derived alkaloid from Sophora flavescens with therapeutic potential for non-small cell lung cancer through ROS-mediated pyroptosis

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Homo sapiens

PMID:

38630006

Title:

Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut-breast axis - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Progress in neuro-psychopharmacology & biological psychiatry

Application:

IF:

5.6

Species:

Mus musculus

PMID:

38484929

Title:

Antidepressant effects of esketamine via the BDNF/AKT/mTOR pathway in mice with postpartum depression and their offspring

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Arteriosclerosis, thrombosis, and vascular biology

Application:

IF:

10.51

Species:

Homo sapiens

PMID:

37881938

Title:

Aggf1 Specifies Hemangioblasts at the Top of Regulatory Hierarchy via Npas4l and mTOR-S6K-Emp2-ERK Signaling

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Chemico-biological interactions

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

38815668

Title:

Sevoflurane enhances autophagy via Rac1 to attenuate lung ischaemiareperfusion injury

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38861809

Title:

Novel Hsp90-Targeting PROTACs: Enhanced Synergy with Cisplatin in Combination Therapy of Cervical Cancer

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

World journal of gastroenterology

Application:

IF:

4.3

Species:

Oryctolagus cuniculus

PMID:

39086630

Title:

Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Rattus norvegicus

PMID:

39004783

Title:

Therapeutic effects of exendin-4 on spinal cord injury via restoring autophagy function and decreasing necroptosis in neuron - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of Cancer

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

38706907

Title:

Knockdown of LRP5 Promotes Proliferation and Invasion of Tongue Squamous Cell Carcinoma through Compensatory Activation of Akt Signaling - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

37884218

Title:

A composition of ursolic acid derivatives from Ludwigia hyssopifolia induces apoptosis in throat cancer cells via the Akt/mTOR and mitochondrial signaling pathways

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

38735265

Title:

Biological impact and therapeutic potential of a novel camptothecin derivative (FLQY2) in pancreatic cancer through inactivation of the PDK1/AKT/mTOR pathway

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

39180780

Title:

Cadmium causes spleen toxicity in chickens by regulating mitochondrial unfolded protein response and nuclear receptors response - PMC

References for Phospho-mTOR-S2448 Rabbit mAb (AP0115)

Product:

Phospho-mTOR-S2448 Rabbit mAb

Journal:

Journal of hazardous materials

Application:

IF:

12.2

Species:

Rattus norvegicus

PMID:

38335616

Title:

The role of mTORC1/TFEB axis mediated lysosomal biogenesis and autophagy impairment in fluoride neurotoxicity and the intervention effects of resveratrol