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Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of C6 cells using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.

Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564) at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.

Review

Overview

Product name	Phospho-p70 S6 Kinase 1-T389 Rabbit pAb
Catalog No.	AP0564
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T389 of human P70 S6K (NP_003152.1).
Sequence	GFTYVA
Gene ID
Swiss Prot
Synonyms	S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; Phospho-p70 S6 Kinase 1-T389
Calculated MW	59kDa
Observed MW	70kDa/85kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by FBS
Cellular location	Cell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome.
Customer validation	(MSC cells, unknow) WB(Homo sapiens, Spodoptera frugiperda, Mus musculus, Sus scrofa, Rattus norvegicus, Danio rerio, M. japonicus, Mus musculus, Drosophila melanogaster, Gallus gallus, Bos taurus) IHC(Mus musculus, Rattus norvegicus) IF(Mus musculus, Rattus norvegicus)

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Research Area

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb images

Western blot - Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Immunofluorescence - Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of C6 cells using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.

Immunofluorescence - Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Immunofluorescence analysis of HeLa cells using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564) at dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37℃.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Biochemical Pharmacology

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

27816546

Title:

Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.016

Species:

Homo sapiens

PMID:

30741586

Title:

Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Virulence

Application:

IF:

5.882

Species:

Spodoptera frugiperda

PMID:

33509041

Title:

Autophagy induced by Vip3Aa has a pro-survival role in Spodoptera frugiperda Sf9 cells

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Neurochemical Research

Application:

IF:

3.996

Species:

Mus musculus

PMID:

31865521

Title:

Autophagy Dysfunction and mTOR Hyperactivation Is Involved in Surgery: Induced Behavioral Deficits in Aged C57BL/6J Mice

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

32508661

Title:

Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Stem Cell Res Ther

Application:

IF:

6.832

Species:

Homo sapiens

PMID:

33579362

Title:

Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Toxicol Lett

Application:

IF:

4.37

Species:

Homo sapiens

PMID:

33781819

Title:

Targeting SLC1A5 blocks cell proliferation through inhibition of mTORC1 in arsenite-treated human uroepithelial cells

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Mol Ther Nucleic Acids

Application:

IF:

8.88

Species:

Mus musculus

PMID:

32585629

Title:

miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Acta Pharm Sin B

Application:

IHC

IF:

11.41

Species:

Mus musculus

PMID:

34900535

Title:

Discovery of a potent FKBP38 agonist that ameliorates HFD-induced hyperlipidemia via mTOR/P70S6K/SREBPs pathway

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

J Cancer

Application:

IF:

4.207

Species:

Mus musculus

PMID:

34659548

Title:

Bai-He-Gu-Jin-Tang formula suppresses lung cancer via AKT/GSK3β/β-catenin and induces autophagy via the AMPK/mTORC1/ULK1 signaling pathway

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

4.55

Species:

Mus musculus

PMID:

34948132

Title:

Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Animal bioscience

Application:

IF:

Species:

Sus scrofa

PMID:

34445847

Title:

Effects of protein content and the inclusion of protein sources with different amino acid release dynamics on the nitrogen utilization of weaned piglets

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Rattus norvegicus

PMID:

34830430

Title:

Synbiotics alleviate hepatic damage, intestinal injury and muscular beclin-1 elevation in rats after chronic ethanol administration

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

PLoS biology

Application:

IF:

9.59

Species:

Danio rerio

PMID:

36318514

Title:

Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1 - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

M. japonicus

PMID:

36067252

Title:

White spot syndrome virus directly activates mTORC1 signaling to facilitate its replication via polymeric immunoglobulin receptor-mediated infection in shrimp - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Journal of endocrinological investigation

Application:

IF:

5.46

Species:

Homo sapiens

PMID:

36306106

Title:

CircNDST1 promotes papillary thyroid cancer progression via its interaction with CSNK2A1 to activate the PI3K–Akt pathway and epithelial–mesenchymal …

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36568155

Title:

HRK inhibits colorectal cancer cells proliferation by suppressing the PI3K/AKT/mTOR pathway - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

The Journal of pathology

Application:

IF:

9.88

Species:

Homo sapiens

PMID:

36373776

Title:

Cancer‐associated fibroblasts potentiate colorectal cancer progression by crosstalk of the IGF2–IGF1R and Hippo–YAP1 signaling pathways

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

Sus scrofa

PMID:

36735752

Title:

Picornavirus infection enhances aspartate by the SLC38A8 transporter to promote viral replication - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

37002971

Title:

Saikosaponin A and D Attenuate Skeletal Muscle Atrophy in Chronic Kidney Disease by Reducing Oxidative Stress through Activation of PI3K/AKT/Nrf2 …

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens, Mus musculus

PMID:

37532694

Title:

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

4.81

Species:

Homo sapiens

PMID:

37934902

Title:

Maackiain suppresses the development of cervical cancer via AMPK priming autophagy

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Food and chemical toxicology

Application:

IF:

5.572

Species:

Homo sapiens

PMID:

30578841

Title:

Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Advanced Science

Application:

IHC

IF:

15.1

Species:

Rattus norvegicus

PMID:

38037294

Title:

LigandScreened CeriumBased MOF Microcapsules Promote Nerve Regeneration via Mitochondrial Energy Supply

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

iScience

Application:

IF:

5.8

Species:

Drosophila melanogaster

PMID:

38292423

Title:

Maintaining Toll signaling in Drosophila brain is required to sustain autophagy for dopamine neuron survival - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38324392

Title:

Bergenin Alleviates Ulcerative Colitis By Decreasing Gut Commensal Bacteroides vulgatus-Mediated Elevated Branched-Chain Amino Acids

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.988

Species:

Homo sapiens

PMID:

35418865

Title:

Ag120-Mediated Inhibition of ASCT2-Dependent Glutamine Transport has an Anti-Tumor Effect on Colorectal Cancer Cells

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

5.037

Species:

Rattus norvegicus

PMID:

32360572

Title:

Hyperoside attenuates pregnancy loss through activating autophagy and suppressing inflammation in a rat model

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.01

Species:

Gallus gallus

PMID:

36621100

Title:

Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1-F3) in late-phase laying hens

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

ACS omega

Application:

IF:

4.1

Species:

Mus musculus

PMID:

38496927

Title:

Identification of Active Components for Sports Supplements: Machine Learning-Driven Classification and Cell-Based Validation

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Rattus norvegicus

PMID:

38611372

Title:

Kaempferol Improves Exercise Performance by Regulating Glucose Uptake, Mitochondrial Biogenesis, and Protein Synthesis via PI3K/AKT and MAPK Signaling Pathways - PMC

References for Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (AP0564)

Product:

Phospho-p70 S6 Kinase 1-T389 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

38840221