제품 > 항체 > 인산화 특이적 항체

Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60S.

ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of HeLa cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of NIH/3T3 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of C6 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunohistochemistry - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namePhospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb
Catalog No.AP0502
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0107
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
ImmunogenA synthetic phosphorylated peptide around T421&S424 of human Phospho S6K1(NP_001258989.1).
SequenceSVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
Gene ID
Swiss Prot
SynonymsS6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha; Phospho-p70 S6 Kinase 1-T421/S424
Calculated MW59kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3 treated by 10% FBS, C6 treated by 10% FBS
Cellular locationCell junction, Cytoplasm, Mitochondrion outer membrane, Mitochondrion, Nucleus, synapse, synaptosome.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens)

IF(Mus musculus)

WB(Mus musculus)

FC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)}

Western blot - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60S.
ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)}

Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of HeLa cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)}

Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of NIH/3T3 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)}

Immunofluorescence - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Confocal imaging of C6 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunohistochemistry - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)}

Immunohistochemistry - Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (AP0502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0502? Please let us know so that we can cite the reference in this datasheet.

항체 (9)

Secondary Antibodies (26)