제품 > 항체 > 인산화 특이적 항체

Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit pAb (AP0767)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit pAb (AP0767)

Western blot analysis of various lysates using (AP0767) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit pAb
Catalog No.AP0767
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenA synthetic phosphorylated peptide around S363 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
SequenceKDSPG
Gene ID
Swiss Prot
SynonymsRSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; Phospho-p90Rsk/RSK1/RPS6KA1-S363
Calculated MW83kDa
Observed MW90kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa treated by PMA
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit pAb (AP0767)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit pAb (AP0767)

Western blot analysis of various lysates using (AP0767) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (6)

Secondary Antibodies (26)