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Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909)

Western blot analysis of various lysates using Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for Proprotein Convertase 9(PCSK9) Rabbit mAb(A21909)

Overview

Product nameProprotein Convertase 9(PCSK9) Rabbit mAb
Catalog No.A21909
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0695
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human Proprotein Convertase 9(PCSK9) (Q8NBP7).
SequenceMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASC
Gene ID
Swiss Prot
SynonymsFH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3; Proprotein Convertase 9(PCSK9)
Calculated MW74kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular locationCell surface, Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Secreted.
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ABclonal:Western blot - Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909)}

Western blot - Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909)

Western blot analysis of various lysates using Proprotein Convertase 9(PCSK9) Rabbit mAb (A21909) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)