제품 > 항체 > 단일클론항체(mAb)

RAB45 Rabbit mAb (A21163)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - RAB45 Rabbit mAb (A21163)

Western blot analysis of various lysates, using RAB45 Rabbit mAb (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - RAB45 Rabbit mAb (A21163)

Western blot analysis of various lysates, using RAB45 Rabbit mAb (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameRAB45 Rabbit mAb
Catalog No.A21163
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3022
This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RAB45 (NP_689786.2).
SequenceMEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCG
Gene ID
Swiss Prot
SynonymsTSG; RAB45
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSK-BR-3, T47D, LNCaP, K-562, Mouse stomach
Cellular locationCytoplasm, perinuclear region

Documents

Certificate of Compliance

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ABclonal:Western blot - RAB45 Rabbit mAb (A21163)}

Western blot - RAB45 Rabbit mAb (A21163)

Western blot analysis of various lysates, using RAB45 Rabbit mAb (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - RAB45 Rabbit mAb (A21163)}

Western blot - RAB45 Rabbit mAb (A21163)

Western blot analysis of various lysates, using RAB45 Rabbit mAb (A21163) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.