제품 > 항체 > 다중클론항체(pAb)

RAD17 Rabbit pAb (A5359)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - RAD17 Rabbit pAb (A5359)

Western blot analysis of various lysates using RAD17 Rabbit pAb (A5359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunoprecipitation - RAD17 Rabbit pAb (A5359)

Immunoprecipitation analysis of 200 μg extracts of K562 cells using 1 μg RAD17 antibody (A5359). Western blot was performed from the immunoprecipitate using RAD17 antibody (A5359) at a dilution of 1:1000.

Overview

Product nameRAD17 Rabbit pAb
Catalog No.A5359
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
SequenceKILYCKRASLTELDSPRLPSHLSEYERDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIPMRNQAQISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT
Gene ID
Swiss Prot
SynonymsCCYC; R24L; RAD24; HRAD17; RAD17SP; RAD17
Calculated MW77kDa
Observed MW77kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesSW480, BT-474, K-562, 22Rv1, Raji
Cellular locationNucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - RAD17 Rabbit pAb (A5359)}

Western blot - RAD17 Rabbit pAb (A5359)

Western blot analysis of various lysates using RAD17 Rabbit pAb (A5359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunoprecipitation - RAD17 Rabbit pAb (A5359)}

Immunoprecipitation - RAD17 Rabbit pAb (A5359)

Immunoprecipitation analysis of 200 μg extracts of K562 cells using 1 μg RAD17 antibody (A5359). Western blot was performed from the immunoprecipitate using RAD17 antibody (A5359) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)