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RBBP5 Rabbit pAb (A6965)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RBBP5 Rabbit pAb (A6965)

Western blot analysis of various lysates using RBBP5 Rabbit pAb (A6965) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Rat Intestine using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RBBP5 Rabbit pAb (A6965)

Immunofluorescence analysis of A-549 cells using RBBP5 Rabbit pAb (A6965). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - RBBP5 Rabbit pAb (A6965)

Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg RBBP5 antibody (A6965). Western blot was performed from the immunoprecipitate using RBBP5 antibody (A6965) at a dilution of 1:1000.

Overview

Product nameRBBP5 Rabbit pAb
Catalog No.A6965
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2).
SequenceSIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALLYLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDGKSKKKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEGSAKGKVQAELSQPLTAGGAISELL
Gene ID
Swiss Prot
SynonymsRBQ3; SWD1; RBBP5
Calculated MW59kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7, NCI-H460, HeLa, Mouse spleen, Mouse brain, Mouse thymus, Rat liver
Cellular locationNucleus
Customer validation

ChIP(Homo sapiens)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - RBBP5 Rabbit pAb (A6965)}

Western blot - RBBP5 Rabbit pAb (A6965)

Western blot analysis of various lysates using RBBP5 Rabbit pAb (A6965) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)}

Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Rat Intestine using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)}

Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RBBP5 Rabbit pAb (A6965)}

Immunohistochemistry - RBBP5 Rabbit pAb (A6965)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using RBBP5 Rabbit pAb (A6965) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RBBP5 Rabbit pAb (A6965)}

Immunofluorescence - RBBP5 Rabbit pAb (A6965)

Immunofluorescence analysis of A-549 cells using RBBP5 Rabbit pAb (A6965). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - RBBP5 Rabbit pAb (A6965)}

Immunoprecipitation - RBBP5 Rabbit pAb (A6965)

Immunoprecipitation analysis of 300 μg extracts of MCF7 cells using 3 μg RBBP5 antibody (A6965). Western blot was performed from the immunoprecipitate using RBBP5 antibody (A6965) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6965? Please let us know so that we can cite the reference in this datasheet.