제품 > 항체 > 다중클론항체(pAb)

RHOF Rabbit pAb (A4786)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - RHOF Rabbit pAb (A4786)

Western blot analysis of various lysates using RHOF Rabbit pAb (A4786) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameRHOF Rabbit pAb
Catalog No.A4786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable GTP binding activity; GTPase activity; and protein kinase binding activity. Involved in actin filament organization. Located in extracellular exosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human RHOF (NP_061907.2).
SequenceSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHF
Gene ID
Swiss Prot
SynonymsRIF; ARHF; RHOF
Calculated MW24kDa
Observed MW27kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, 293T, Mouse kidney
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, cytoskeleton

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ABclonal:Western blot - RHOF Rabbit pAb (A4786)}

Western blot - RHOF Rabbit pAb (A4786)

Western blot analysis of various lysates using RHOF Rabbit pAb (A4786) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)